Multi-Mode Mimetic Ligand™ Library

Looking back over the last 30 years downstream processes have changed significantly. Traditionally purification processes consisted of a series of steps which provided progressive increases in purity until eventually the desired purity was achieved. However this approach involves many unit operations which contributes to increased costs and reduced yields.
 
In comparison, modern downstream processes make use of affinity capture steps to provide a large increase in purity at the first step, which makes removal of residual impurities relatively straightforward by use of one or more polishing steps where the impurities are captured and the product passes straight through. Today most monoclonal antibodies are purified using this approach using immobilized Protein A as an affinity capture step.
 
Multi-mode ligands have grown to prominence in recent years due to their usefulness as general ligands for capture and polishing applications. These ligands combine a charge group and a hydrophobic group into one ligand structure.
 
Binding and elution is performed by tuning the buffer conditions to favour protein adsorption or desorption. This is achieved by addition or removal of salts and/or the addition or removal of polarity reducing agents such as alcohols or glycols.
 
However, there is currently a significant limitation to the application of multi-mode ligands in that relatively few of these ligands are currently available commercially. As a consequence, buffer conditions are optimized to suit the ligands available -  which may not always be compatible with the protein in question.
 
We believe a better approach to multi-mode ligand chromatography is to produce a diverse array of multi-mode ligands and select the ligand that has binding and elution properties compatible with the target protein and feedstock.Looking back over the last 30 years downstream processes have changed significantly. Traditionally purification processes consisted of a series of steps which provided progressive increases in purity until eventually the desired purity was achieved. However this approach involves many unit operations which contributes to increased costs and reduced yields.

To purchase the Multi-mode Mimetic Ligand™  Library

Evolution of Downstream Processes